Equipment
Axon MultiClamp 700B Amplifier
A versatile, computer-controlled microelectrode amplifier designed for patch voltage-clamp or high-speed current-clamp recording within the same headstage ideal for many applications, including high-speed current clamp (sharp-electrode or field potentials), patch-clamp (whole-cell, macropatch, or excised patch), ion-selective measurements, etc. New features include internal automatic mode switching, oscillation suppression for better cell protection, and slow current injection to compensate for drifting potentials.
Slice Scope Pro 1000
A compact upright microscope equipped with fully motorized fixed stage, various electrode manipulators, a wide range of Olympus objectives and LED system (780 nm) to meet the demands of electrophysiology study.
Slice Scope Pro 3000
A static microscope designed for in vitro electrophysiology and capable of accommodating advanced imaging, such as two-photon and confocal. The system consists of a SliceScope microscope and motorized movable top plate and two PatchStar micromanipulators.
Digidata 1550A Digitizer
Most widely used digitizer for a wide variety of electrophysiology recordings in laboratories worldwide. It further extends the precision and quality of the existing Axon Digitizer platform with enhanced performance in command voltage output control and sampling rate.
TDT 128 Channel Neurophysiology System
Designed for everything from moths to man, record anywhere up to 128 channels. Stream all channels to disk at full bandwidth. Conduct real-time spike sorting and local field potentials recording. It consists of 3 major components: RZ2 BioAmp processor, RS4 Data Streamer, and PZ5-128 NeuroDigitizer. Preinstalled Synapse Software Suite automatically synchronizes all data inputs in a perfectly timed manner: spike data, local field potentials epoch events, and control signal.
P-340 fura2
The PE-340 fura2 LED illumination system provides the optimum excitation wavelengths for Fura-2 based calcium imaging, allowing high-precision, stable, high -throughput imaging with video-rate time resolution.
Sutter P-2000
Sutter P2000 micropipette puller represents a significant advance in the technology of fabrication of micropipettes, optical fiber probes and nano-spray tips. P2000 integrates a CO2 laser_based heat source with the technology derived from extensive experience with conventional pullers.
Main features of Sutter P-2000 (SU P2000) Glass Puller:
- Capable of pulling quartz, borosilicate and aluminosilicate glass
- Pulls electrodes with tip diameters smaller than 0.03 um
- Preprogrammed sample programs for intra-cellular and patch pipettes
Pipettes made from SU P2000:
Conventional patching pipettes
microinjection pipettes
SECM image of cleaned pipettes
Benefits:
Optimized velocity sensing circuit for maximized sensitivity and reproducibility
Operating life of the CO2 laser is expected to be over 10 years
Applications:
Patch clamp
Intracellular recording,
Microinjection
Nano probe research
SECM